Proteome analysis of apical and basal regions of poplar stems under gravitropic stimulation.
Identifieur interne : 003514 ( Main/Exploration ); précédent : 003513; suivant : 003515Proteome analysis of apical and basal regions of poplar stems under gravitropic stimulation.
Auteurs : Wassim Azri [France] ; Christophe Chambon ; Stéphane Herbette ; Nicole Brunel ; Catherine Coutand ; Jean-Charles Leplé ; Ichrak Ben Rejeb ; Saïda Ammar ; Jean-Louis Julien ; Patricia Roeckel-DrevetSource :
- Physiologia plantarum [ 1399-3054 ] ; 2009.
Descripteurs français
- KwdFr :
- Gravitropisme (physiologie), Populus (métabolisme), Populus (physiologie), Protéines végétales (métabolisme), Protéomique (MeSH), Régulation de l'expression des gènes végétaux (MeSH), Spectrométrie de masse MALDI (MeSH), Spectrométrie de masse en tandem (MeSH), Tiges de plante (métabolisme), Tiges de plante (physiologie), Électrophorèse bidimensionnelle sur gel (MeSH).
- MESH :
English descriptors
- KwdEn :
- Electrophoresis, Gel, Two-Dimensional (MeSH), Gene Expression Regulation, Plant (MeSH), Gravitropism (physiology), Plant Proteins (metabolism), Plant Stems (metabolism), Plant Stems (physiology), Populus (metabolism), Populus (physiology), Proteomics (MeSH), Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (MeSH), Tandem Mass Spectrometry (MeSH).
- MESH :
- chemical , metabolism : Plant Proteins.
- metabolism : Plant Stems, Populus.
- physiology : Gravitropism, Plant Stems, Populus.
- Electrophoresis, Gel, Two-Dimensional, Gene Expression Regulation, Plant, Proteomics, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Tandem Mass Spectrometry.
Abstract
Gravity is a constant force guiding the direction of plant growth. In young poplar stem, reorientation of the apical region is mainly obtained by differential growth of elongating primary tissues. At the base, where elongation is achieved but where the cambium is active, reorientation is due to asymmetrical formation of reaction wood. After 45 min of gravistimulation, the stem showed no reorientation, but 1 week later, reaction wood was observed at the base of the stem. To determine the molecular mechanisms taking place at the top and base of the stem, after 45 min or 1 week of inclination, the changes induced in protein accumulation were studied by two-dimensional polyacrylamide gel electrophoresis and quantitatively analyzed using image analysis software. Around 300 protein spots were reproducibly detected and analyzed. Forty percent of these proteins showed significant changes after inclination. Mass spectrometry analysis of 135 spots led to the identification of 60 proteins involved in a wide range of activities and metabolisms. Very different patterns of protein expression were obtained according to conditions tested, highlighting the complexity of gravitropic responses. Our results suggest that primary and secondary tissues present specific mechanisms to sense reorientation and to respond to inclination. Some selected proteins are discussed.
DOI: 10.1111/j.1399-3054.2009.01230.x
PubMed: 19453506
Affiliations:
Links toward previous steps (curation, corpus...)
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In young poplar stem, reorientation of the apical region is mainly obtained by differential growth of elongating primary tissues. At the base, where elongation is achieved but where the cambium is active, reorientation is due to asymmetrical formation of reaction wood. After 45 min of gravistimulation, the stem showed no reorientation, but 1 week later, reaction wood was observed at the base of the stem. To determine the molecular mechanisms taking place at the top and base of the stem, after 45 min or 1 week of inclination, the changes induced in protein accumulation were studied by two-dimensional polyacrylamide gel electrophoresis and quantitatively analyzed using image analysis software. Around 300 protein spots were reproducibly detected and analyzed. Forty percent of these proteins showed significant changes after inclination. Mass spectrometry analysis of 135 spots led to the identification of 60 proteins involved in a wide range of activities and metabolisms. Very different patterns of protein expression were obtained according to conditions tested, highlighting the complexity of gravitropic responses. Our results suggest that primary and secondary tissues present specific mechanisms to sense reorientation and to respond to inclination. Some selected proteins are discussed.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19453506</PMID><DateCompleted><Year>2009</Year><Month>07</Month><Day>09</Day></DateCompleted><DateRevised><Year>2009</Year><Month>06</Month><Day>03</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1399-3054</ISSN><JournalIssue CitedMedium="Internet"><Volume>136</Volume><Issue>2</Issue><PubDate><Year>2009</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Physiologia plantarum</Title><ISOAbbreviation>Physiol Plant</ISOAbbreviation></Journal><ArticleTitle>Proteome analysis of apical and basal regions of poplar stems under gravitropic stimulation.</ArticleTitle><Pagination><MedlinePgn>193-208</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1399-3054.2009.01230.x</ELocationID><Abstract><AbstractText>Gravity is a constant force guiding the direction of plant growth. In young poplar stem, reorientation of the apical region is mainly obtained by differential growth of elongating primary tissues. At the base, where elongation is achieved but where the cambium is active, reorientation is due to asymmetrical formation of reaction wood. After 45 min of gravistimulation, the stem showed no reorientation, but 1 week later, reaction wood was observed at the base of the stem. To determine the molecular mechanisms taking place at the top and base of the stem, after 45 min or 1 week of inclination, the changes induced in protein accumulation were studied by two-dimensional polyacrylamide gel electrophoresis and quantitatively analyzed using image analysis software. Around 300 protein spots were reproducibly detected and analyzed. Forty percent of these proteins showed significant changes after inclination. Mass spectrometry analysis of 135 spots led to the identification of 60 proteins involved in a wide range of activities and metabolisms. Very different patterns of protein expression were obtained according to conditions tested, highlighting the complexity of gravitropic responses. Our results suggest that primary and secondary tissues present specific mechanisms to sense reorientation and to respond to inclination. Some selected proteins are discussed.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Azri</LastName><ForeName>Wassim</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Université Blaise Pascal, UMR547 PIAF, 24 av des Landais, F-63177 Aubière, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chambon</LastName><ForeName>Christophe</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Herbette</LastName><ForeName>Stéphane</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Brunel</LastName><ForeName>Nicole</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Coutand</LastName><ForeName>Catherine</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Leplé</LastName><ForeName>Jean-Charles</ForeName><Initials>JC</Initials></Author><Author ValidYN="Y"><LastName>Ben Rejeb</LastName><ForeName>Ichrak</ForeName><Initials>I</Initials></Author><Author ValidYN="Y"><LastName>Ammar</LastName><ForeName>Saïda</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Julien</LastName><ForeName>Jean-Louis</ForeName><Initials>JL</Initials></Author><Author ValidYN="Y"><LastName>Roeckel-Drevet</LastName><ForeName>Patricia</ForeName><Initials>P</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>03</Month><Day>11</Day></ArticleDate></Article><MedlineJournalInfo><Country>Denmark</Country><MedlineTA>Physiol Plant</MedlineTA><NlmUniqueID>1256322</NlmUniqueID><ISSNLinking>0031-9317</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D015180" MajorTopicYN="N">Electrophoresis, Gel, Two-Dimensional</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018522" MajorTopicYN="N">Gravitropism</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018547" MajorTopicYN="N">Plant Stems</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D040901" MajorTopicYN="Y">Proteomics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019032" MajorTopicYN="N">Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053719" MajorTopicYN="N">Tandem Mass Spectrometry</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>5</Month><Day>21</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>5</Month><Day>21</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>7</Month><Day>10</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19453506</ArticleId><ArticleId IdType="pii">PPL1230</ArticleId><ArticleId IdType="doi">10.1111/j.1399-3054.2009.01230.x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li></country><region><li>Auvergne (région administrative)</li><li>Auvergne-Rhône-Alpes</li></region><settlement><li>Aubière</li></settlement><orgName><li>Université Blaise-Pascal</li></orgName></list><tree><noCountry><name sortKey="Ammar, Saida" sort="Ammar, Saida" uniqKey="Ammar S" first="Saïda" last="Ammar">Saïda Ammar</name><name sortKey="Ben Rejeb, Ichrak" sort="Ben Rejeb, Ichrak" uniqKey="Ben Rejeb I" first="Ichrak" last="Ben Rejeb">Ichrak Ben Rejeb</name><name sortKey="Brunel, Nicole" sort="Brunel, Nicole" uniqKey="Brunel N" first="Nicole" last="Brunel">Nicole Brunel</name><name sortKey="Chambon, Christophe" sort="Chambon, Christophe" uniqKey="Chambon C" first="Christophe" last="Chambon">Christophe Chambon</name><name sortKey="Coutand, Catherine" sort="Coutand, Catherine" uniqKey="Coutand C" first="Catherine" last="Coutand">Catherine Coutand</name><name sortKey="Herbette, Stephane" sort="Herbette, Stephane" uniqKey="Herbette S" first="Stéphane" last="Herbette">Stéphane Herbette</name><name sortKey="Julien, Jean Louis" sort="Julien, Jean Louis" uniqKey="Julien J" first="Jean-Louis" last="Julien">Jean-Louis Julien</name><name sortKey="Leple, Jean Charles" sort="Leple, Jean Charles" uniqKey="Leple J" first="Jean-Charles" last="Leplé">Jean-Charles Leplé</name><name sortKey="Roeckel Drevet, Patricia" sort="Roeckel Drevet, Patricia" uniqKey="Roeckel Drevet P" first="Patricia" last="Roeckel-Drevet">Patricia Roeckel-Drevet</name></noCountry><country name="France"><region name="Auvergne-Rhône-Alpes"><name sortKey="Azri, Wassim" sort="Azri, Wassim" uniqKey="Azri W" first="Wassim" last="Azri">Wassim Azri</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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